Cell Culture
We produce cell culture media in various formats, with or without serum, and containing different types of supplements to support several cell types and applications. Choose from chemically-defined, animal component-free, and classical cell culture media to support the optimal growth of your cells.
Chemically Defined Media
This media is for in vitro human or animal cell culture. As we optimize all the chemical components this type of media is suitable for industrial projects where reproducibility is crucial.
Animal Component Free Media
The animal component media is suitable for human cell culture such as drug testing, mechanism of action studies or cellular differentiation or for adapting for therapeutic applications.
Classical Cell Culture Media
The classical media composition may contain serum or components to allow long term culture or for general cell-based assays or vitro studies.
Cell Culture Handbook
Cell culture is a method used in biological research to isolate fragments of cells or tissues from an organism and subsequently grow them in a favourable artificial environment. Cell culture laboratories present several specific hazards associated with cell and tissue manipulation, as well as toxic, corrosive, or mutagenic solvents and reagents. To ensure an appropriate biosafety level, it is necessary to strictly adhere to standard microbiological practices and techniques.
Every cell culture laboratory, regardless of the type of samples handled, requires it to be free from pathogenic microorganisms (i.e., asepsis) and share some of the same basic equipment that is essential for culturing cells. Laminar flow hoods are ventilation devices used within cell culture labs to provide an aseptic work area that helps protect both the personnel and the materials they are working with. Cell culture hoods are used to create a sterile environment and to protect the samples from contamination. Essential in any cell culture lab is the incubator, a piece of equipment that allows cells and tissues to be protected in a sealed chamber with strictly regulated conditions, including temperature, humidity, CO2 and O2. Equally important are the pipettes and tools used to dispense accurate and measured volumes of liquids. They can be categorised as micropipettes (that dispense between 1 and 1000µl) and macro pipettes (that dispense a volume greater than 1000µl). Pipettes come in several designs, including manual and electronic, as well as single-channel or multi-channel pipettes. Finally, another essential component is the microscope, needed to examine cell growth (confluency and morphology), and cell proliferation, as well as to ensure that the culture is free from contamination.
One of the most important components of the cell culture environment is the cell medium that provides the necessary elements to support cell growth, including nutrients, hormones, and growth factors. The culture medium also regulates the pH and the osmotic pressure of the culture. Furthermore, different types of plastic vessels can be used for cell culture and cell-based assays. Cell culture plates (including 6-, 12-, 24- and 96-well plates), dishes and flasks (such as T-25) are available.
Cell Culture Essentials
Cell culture media contain several components to ensure cell survival and growth. These include glucose, L-glutamine and serum.
Advantages Of Stemnovate's ETFDS
| Glucose | Glutamine | Serum | Hepes | Sodium pyruvate | Phenol red |
|---|---|---|---|---|---|
| High | GlutaMAX | Standard | Yes | Yes | Yes |
| Low | L-glutamine | Reduced serum | No | No | No |
| None | - | no serum | - | - | - |
Useful Cell Culture Numbers
| - | Surface area (cm2) | Seeding density * | Cells at confluency+ | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA) | Cell medium required (mL) |
|---|---|---|---|---|---|
| FLASKS | |||||
| T-25 | 25 | 0.7 x 106 | 2.8 x 106 | 3 | 3–5 |
| T-75 | 75 | 2.1 x 106 | 8.4 x 106 | 5 | 8–15 |
| T-175 | 175 | 4.9 x 106 | 23.3 x 106 | 17 | 35–53 |
| T-225 | 225 | 6.3 x 106 | 30 x 106 | 22 | 45–68 |
| CULTURE PLATES | |||||
| 6-well | 9.6 | 0.3 x 106 | 1.2 x 106 | 1 | 1 to 3 |
| 12-well | 3.5 | 0.1 x 106 | 0.5 x 106 | 0.4 to 1 | 1 to 2 |
| 24-well | 1.9 | 0.05 x 106 | 0.24 x 106 | 0.2 to 0.3 | 0.5 to 1.0 |
| 48-well | 1.1 | 0.03 x 106 | 0.12 x 106 | 0.1 to 0.2 | 0.2 to 0.4 |
| 96-well | 0.32 | 0.01 x 106 | 0.04 x 106 | 0.05 to 0.1 | 0.1 to 0.2 |
| DISHES | |||||
| 35mm | 8.8 | 0.04 x 106 | 1.2 x 106 | 1 | 2 |
| 60mm | 21.5 | 0.8 x 106 | 3.2 x 106 | 3 | 5 |
| 100mm | 56.7 | 2.2 x 106 | 8.8 x 106 | 5 | 12 |
| 150mm | 145 | 5.0 x 106 | 20.0 x 106 | 10 | 30 |
+ The number of cells on a confluent plate, dish, or flask will vary with cell type (HeLa cells were used).
Work With Us!
At Stemnovate we have developed ready-to-use cell culture media, but we can also customize your media to fit your project (and your budget). We can provide technical support recommendations for your cell culture, including tips for experimental setup and in-depth troubleshooting help.