Primary Canine Fibroblasts (SV-CF-4-500-Live)

Stemnovate’s Primary Canine Fibroblasts (SV-CF-4-500-L) are created by isolating, plating, and expanding cells in culture before being harvested for cryopreservation, resulting in the highest purity, viability, and plating efficiency. The key features of these primary canine fibroblasts (SV-CF-4-500-L) are:

• Highly proliferative and healthy (~90% viability)
• High cell counts (~0.5 million cells per vial)
• Ideal for 2D and 3D cultures, microfluidic devices, co-cultures, tissue engineering, and skin models
• Available as single vials or reserved lots

Product Testing

Stemnovate’s Primary Canine Fibroblasts (SV-CF-4-500-L) are negative for tuberculosis and mycoplasma. Our recommendation is not to freeze and thaw the cells repeatedly. Additional options are available for genotyping for drug-metabolizing enzymes along with tailored media to meet custom research needs. 

Stemnovate’s Canine Primary Dermal Fibroblasts have many applications, for example:

• In the assay of cell-cell interaction, adhesion, PCR, laminin, and fibronectin production
• Studying fibroblast's growth factor response
• In immunofluorescent flow cytometry
• Generating cell derivatives for desired research applications.

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin and play a crucial role during wound healing. For instance, they play an important role in the progression of inflammation by secreting various factors that define the tissue microenvironment and modulate immune cell functions.

Dermal fibroblasts provide an excellent model system to study many aspects of cell physiology. For instance, they are integral to hundreds of research publications related to skin biology and reprogramming/induced pluripotency studies.

Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009) for using Human Dermal Fibroblasts in order to create induced pluripotent stem cells (iPSC) by using Oct3/4, Sox2, Klf4, and c-Myc.

All animal cells used by Stemnovate are acquired with consent from the owner, accompanied by a veterinary certificate and are sourced only from necessary surgeries.

Stemnovate products are for in-vitro research only and not to be used for any therapeutics in human or animals.