<p><strong>IN 2020 IN GREAT BRITAIN, 2.88 MILLION PROCEDURES INVOLVED LIVING ANIMALS.</strong>&nbsp;But over half, 53% of experimental testing, was for basic research focusing on the immune system, nervous system and cancers.</p>
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<p>In 2014 the first functional neurons were reported from horses by Sharma et al. Now, our CEO Dr Ruchi leads an industrial partnership creating multi-species neuronal models. These are significant as they provide an alternative to animal testing and would be game-changing for pain management and finding new anaesthetics for veterinary applications. However, the method used for reprogramming stem cells from animal species needs optimisation for cellular therapies, differentiation, and veterinary applications.</p>
<p><br><a class="font-weight-bold" href="https://www.sciencedaily.com/releases/2014/03/140304125833.htm" target="_blank" rel="noopener noreferrer">Read More<span class="visually-hidden">Read more about the scientific discovery on ScienceDaily</span></a></p>
<p class="mt-2">The industrial applications include novel modelling of the brain, liver, and heart for multi-species modelling, replacing in vivo animal testing.</p>

<p>Our team has over ten years of experience creating unique stem cell models that can reduce animal testing.</p>

<p>However, It requires a laboratory assay that consists of injecting cells under mice's skin, allowing cells to differentiate spontaneously and is a determinant of stemness and capability to form multiple cell types. We have integrated stem cell research with tissue engineering, advanced imaging, and molecular analysis, allowing similar data to be generated in the laboratory without animal experiments. This technology further allows fast turnaround, saving costs and unnecessary experimentation.</p>

<p>Stem cells hold great potential for cellular therapies, drug discovery and disease modelling.</p>

<p class="">However, as there is little alternative, animal testing continues.</p>
<p class="mt-2">We are creating new laboratory models to study complex diseases for which it is difficult to obtain human cells, for instance, new treatment development for hearing loss. Neurodegeneration is a common cause of hearing loss. Nevertheless, there are no treatments available. That is why research should focus on novel therapies. Traditionally, foetal mice are sacrificed to obtain the necessary sensory neurons to investigate the disease mechanism.</p>
<p class="mt-2">We are actively working on creating sensory neurons to model hearing loss and encourage research. We initially partnered with Prof Bence at the University of Cambridge to develop a proof of principle, followed by a multinational partnership to explore new methods to create sensory neurons. This first of its kind project can benefit not only patients but could also replace foetal mice usage for research.</p>

<p>Animal models often fail to predict human responses due to interspecies differences.</p>

<p>Pioneering Animal Stem Cell Technology.</p>
<p>We are creating first in class models for dogs, cats, cows, horses and pigs.</p>
<p>Animal research has been an essential contributor to improved human health. We owe several vaccinations and understanding of diseases and treatments to animal sacrifices. But there is an urgent need to reconsider and reduce animal testing.</p>

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<div>In the UK, research involving cats is subject to strict regulations, reserved only for the most critical studies of the nervous and immune systems, or for essential medicine safety testing. Yet even with these controls, in 2024, there were still 82 procedures involving 35 cats.&nbsp;</div>
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<div>We are committed to ending the need for animal testing. But to truly make a difference, the scientific community needs a reliable, reproducible, and thoroughly validated model system&mdash;one that earns the trust of regulators and researchers alike.</div>
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<div>Today, we offer a real, actionable alternative to neuroscience research on cats. Our pioneering neuronal model, developed using a ground-breaking non-integrating, virus-free method&mdash;the first in the world&mdash;sets a new standard for humane and advanced research.&nbsp;Using tissue obtained ethically from routine veterinary procedures, we reprogrammed cells to generate induced pluripotent stem cells. The cat returns home&mdash;healthy, playful, and unharmed&mdash;while its cells help advance science for countless others.</div>
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<div>The cells in our lab acquired the ability to form several cell types.&nbsp;We know this because we conducted in-depth gene analysis using the most advanced sequencing method, studying thousands of genes.</div>
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<div>The feline laboratory stem cells have been in our lab for years now.&nbsp;Further, we directed these to become neurons and then characterised them again. But for any model to be effective, it should function, and neurons are of &nbsp;multiple types with unique properties that depend on the kinds of neurons that have been formed.</div>
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<div>The cats are usually used for sensory function studies, so we created peripheral sensory neurons.&nbsp; As this process is virus-free, it can also be run in parallel to study infectious diseases. And data are made more robust through metagenomic analysis, which uses sequencing to analyze diverse microbes and their cellular functional interactions.</div>
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<div>Today, we unveil the world&rsquo;s first feline data platform that doesn&rsquo;t just mine old data, but generates real-time, robust, and reproducible information&mdash;empowering researchers to make confident, ethical choices.&nbsp;We have commercially provided feline stem cell-derived neuronal technology to a leading animal health company, and now, as a data platform, it is accessible to the wider community.</div>
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<div>Generation of cell lines is expensive because it is niche, requires advanced methods for reprogramming, characterisation with sequencing, bioinformatics, and computation, and that all takes time.</div>
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<div>Now, researchers can accelerate their work with immediate access to our living models&mdash;exploring complex interactions and progressing discoveries faster than ever, all while advancing a humane approach to science.&nbsp;And as this model has been developed and studied commercially over the years, you can explore in-depth science with us and contribute to new drug discovery for both humans and animals.</div>

<p>Stemnovate Animal Data Platform: A Humane Path Beyond Cat Testing for Neuroscience.</p>
<h3><a href="https://stemnovate.co.uk/contact-us" target="_blank" rel="noopener"><span style="color: rgb(230, 126, 35);"><strong>Request a quote</strong></span></a></h3>

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<p>The resulting pain and inflammation in a pet's mouth can hinder their ability to eat or drink, and the bacteria can enter their bloodstream, causing damage to their kidneys and other vital organs. This underscores the critical need for proactive oral health care for your pet.</p>
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<p>Cats and dogs clean their teeth in the wild by chewing on bones or grass, but domestic pets often lack suitable alternatives. Owners may find it challenging to check their pets' oral health, so we are introducing the most advanced technology to help ensure the oral health of beloved pets. Our advanced technology equip veterinarians and owners to know about the microbes affecting pets.<br><br></p>
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<p>The DEN-OR-KIT is not just a kit; it's a game-changer for your pet's oral health. This user-friendly at-home kit offers a comprehensive and accurate analysis of the microbes in your pet's mouth, allowing you to assess their oral and dental health.</p>
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<p>It utilizes advanced DNA sequencing technology and bioinformatic analysis to rapidly profile the oral microbiome. Our cutting-edge analysis method provides a thorough, unbiased test. It covers a broader range of microbes than the quantitative PCR method. Our next-generation sequencing (NGS) approach will comprehensively identify and report the microbes in the provided samples, giving you the power to take control of your pet's oral health.</p>
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<p><a href="https://youtu.be/2hO7-VOXmBE?si=oKZEl0io-ebjpY-W" target="_blank" rel="noopener"><strong id="docs-internal-guid-1ef7c8b1-7fff-4adc-782d-91e3be366b1e">Discover our Oral health test for animals by clicking the link below. Don&rsquo;t forget to subscribe to our channel and connect with us on social media for the latest updates and insights.</strong></a></p>

<p>Left unchecked, pets can develop harmful bacteria, debris, and plaque on their teeth from their food. Over time, this can solidify into tartar, leading to gum irritation, gingivitis, and tooth loss.</p>

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<p><strong>Veterinary Tissue Donation</strong></p>
<p>Imagine that your next visit to the vet could be a meaningful step toward reducing animal testing and enhancing animal welfare.</p>

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<p><strong>Stemnovate&rsquo;s breed identification platform highlights</strong></p>
<p>1. Microsatellite and SNP profiling of cell line.<br>2. Breed identification and percentage composition at a high accuracy.</p>
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<p><span style="color: rgb(230, 126, 35);"><strong>FAQ BREED IDENTIFICATION</strong></span></p>
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<p><span style="color: rgb(45, 194, 107);"><strong>What is a Microsatellite and Single Nucleotide polymorphism (SNP)?</strong></span></p>
<p>Microsatellites or Short tandem repeats (STRs) are DNA sequences that have a repetitive unit of 1-6base pairs which forms lengths of up to 150 nucleotides. Single nucleotide polymorphism (SNPs) is a genomic variant at a single base position in the DNA. SNPs are biological markers that can be used to locate genes. STRs and SNPs are widely spread throughout the genome and are located in the coding and noncoding regions of DNA. They vary slightly among chromosomes e.g. in humans, chromosome 19 has the highest density of STRs. STRs play an important role in forensic science, evolutionary biology, population genetics, QLT mapping, evolutionary genetic and pathogenesis.</p>
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<p><span style="color: rgb(45, 194, 107);"><strong>2.Why is breed identification important?</strong></span></p>
<p>Breed identification can be used to assess breeding history and breed purity of specific animal breeds. It also helps in safeguarding the rights of consumers and producers to prevent the sale and consumption of adulterated products (Dalvit et al., 2007; Sentandreu &amp;amp; Sentandreu, 2014). It also helps with the traceability and conservation efforts of target breeds of interest.</p>
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<p><span style="color: rgb(45, 194, 107);"><strong>3. How does it work?</strong></span></p>
<p>High molecular weight DNA is isolated from the cell line and the genomic DNA (gDNA) is enzymatically digested and tagged with sequencing adaptors on the 5&rsquo; phosphate end and 3&rsquo; hydroxyl group end of the gDNA. &nbsp;Barcoded adapter tagged gDNAs are then transferred to a<br>flowcell for accurate long read sequencing and basecalling. Nucleotide raw reads which can&nbsp;be in either a FASTQ, FASTA or BAM format are computationally assembled and analysed for&nbsp;STR and SNP markers. Detected biomarker polynucleotide regions are ascribed to reference<br>genome database of target breeds at a high accuracy and confidence level.</p>
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<p><strong><span style="color: rgb(45, 194, 107);">4. How long does it take to obtain a result?</span></strong></p>
<p>Turnaround time (TAT) is 6-8 working days</p>

<p>Stemnovate has recently introduced a new service for businesses and researchers working in animal health. This service offers advanced breed identification and bioinformatics to determine breed and gender accurately. Our breed identification service can provide the answers you need to comprehend species' and animal breeds' unique characteristics. Improved understanding can lead to better treatment for your furry friend. Take advantage of this opportunity to learn more - contact us today!</p>

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<p><strong>The primary focus of technology is obtaining detailed information on chromosomal structural variance (SVs) <span style="color: rgb(230, 126, 35);">to identify genomic abnormalities, including deletion, insertion, duplication, and inversion events</span>, without any bias.</strong></p>
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<p><strong>Frequently Asked Questions (FAQ) about Animal-Based Digital Karyotyping:</strong></p>
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<p>1.&nbsp;<strong>What is the animal digital karyotyping platform?</strong></p>
<p>For animal cell line analysis, Stemnovate uses a digital karyotyping tool that screens and identifies structural chromosomal variations such as deletion, duplication, insertion, and translocation changes at the genome level. This platform provides unprecedented access to crucial genetic information for studying cell line stability in cultures, animal diseases and modelling.</p>
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<p>2.&nbsp;<strong>Why is karyotyping important?</strong></p>
<p>Karyotyping detects the chromosomal integrity of cell lines. It's crucial as culturing can compromise it. Factors like cultural media composition conditions can affect cells. It is valuable for identifying animal diseases and structural variations in the animal genome that have applications in animal health drug discovery and breeding.</p>
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<p><strong>3. How does it work?</strong></p>
<p>We culture cells and isolate DNA from cell lines subjected to sequencing, followed by bioinformatic analysis to detect changes in the genetic makeup.</p>
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<p><strong>4. What animal species can be analysed?</strong></p>
<p>We analyse pigs, cats, dogs, horses, chickens, mice, cows, Sheep and C.elegans and zebrafish.&nbsp;<strong>Stemnovate is expanding its animal-based platform to include a broader collection of other animal species.</strong></p>
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<p><strong>5. What is required for the animal digital karyotyping test?</strong></p>
<p>We typically require 1 million cells in a frozen pellet format. We can also culture cells if repeat analysis is required to study batch stability and variations.</p>
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<p><strong>6. How long does it take to obtain a result?</strong></p>
<p>The turnaround time is 9-11 working days. It can be customised to meet specific requirements.</p>
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<p><a href="https://youtu.be/9Halo8PV20U?si=CIRc5EtsklTz18CL" target="_blank" rel="noopener"><strong id="docs-internal-guid-4456c1fd-7fff-f70f-f7a6-63869e8add10">Discover our Digital Karyotyping for Animal species by clicking the link below. Don&rsquo;t forget to subscribe to our channel and connect with us on social media for the latest updates and insights.</strong></a></p>

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<p>Stemnovate has launched a digital Karyotyping service for researchers and businesses in the animal health sector and emerging new technologies such as cultured meat. The platform provides crucial information concerning the genomic integrity of cultured animal cell lines, including primary cells, stem cells, and differentiated cell types.&nbsp;</p>

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<p><a href="https://youtu.be/S1xjv0l4hrw?si=AFMARU6gL1e8MgCN" target="_blank" rel="noopener"><strong id="docs-internal-guid-ccf7f8ac-7fff-b798-594c-dea49118cf19">Learn about our mission and initiatives aimed at reducing animal testing. We invite you to subscribe to our channel and follow us on social media for updates and insights.</strong></a></p>

<p>At Stemnovate, we are dedicated to transforming animal health through innovative genetics and a pioneering technology platform. Our strict no-animal-testing policy guarantees a brighter future for animal-friendly research.</p>

Digital Karyotyping-Animal species DNA Testing

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Oral health test for animals

Partner with Us to Reduce Animal Testing

Breed identification

Digital Karyotyping-Animal species DNA Testing

Animal Platform

Animal Platform

New Insights Platform - Animal Health

Feline Data Platform

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Oral health test for animals

Partner with Us to Reduce Animal Testing

Breed identification

Digital Karyotyping-Animal species DNA Testing

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